منابع مشابه
The Glucose Transporter of Escherichia coli
The glucose transporter of the bacterial phosphotransferase system couples vectorial translocation to phosphorylation of the transported sugar. It consists of a transmembrane subunit (IICBGIc) and a hydrophilic subunit (IIAG1"). The IICBGIC subunit consists of two domains. The NH,-terminal IIC domain (residues 1-386) spans the membrane eight times and contains the substrate binding site. The CO...
متن کاملExpression of the human erythrocyte glucose transporter in Escherichia coli.
The gene encoding the human erythrocyte glucose transporter, cloned from HepG2 hepatoma cells, was expressed in Escherichia coli by introducing a prokaryote-type ribosome binding site, subcloning the gene into the T7 promoter/T7 polymerase expression system, and transforming a strain that is defective in glucose transport. Cells bearing plasmids with the transporter gene take up 2-deoxy-D-gluco...
متن کاملCulture optimization ofEscherichia coli for expression ofgE proteinfrombovine herpesvirus 1 and 5
Background The use of Escherichia coli for the production of recombinant proteins is an established strategy to obtain biotechnological tools. However, the recombinant protein expression is dependent on temperature, bacterial culture time, induction period, nutrients, plasmid characteristics, and insert itself. Thus, the aim of this study was optimize the expression of recombinant gE protein (r...
متن کاملGlucose transporter expression in the human colon
AIM To investigate by immunostaining glucose transporter expression in human colorectal mucosa in controls and patients with inflammatory bowel disease (IBD). METHODS Colorectal samples were obtained from patients undergoing lower endoscopic colonoscopy or recto-sigmoidoscopy. Patients diagnosed with ulcerative colitis (n = 18) or Crohn's disease (n = 10) and scheduled for diagnostic colonosc...
متن کاملThe ascorbate transporter of Escherichia coli.
The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. We show that all three proteins as well as the energy-coupling PTS proteins, enzyme I and HPr, are required for the anaerobic utilization and uptake of L-ascorbate in vi...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)81542-8